Genomic Sequence Lab
| gen677lab1-31-13.doc |
Things you will do today:
1. Find your primary literature article on your gene and disease on PubMed
2. Find your accession numbers and FASTA formats for your DNA and Protein via ENTREZ. You will hyperlink this data out.
3. Determine what Chromosome your gene is located on?
4. Get used to BLAST by going through a tutorial
5. Determine how similar your DNA/protein is to other genes/proteins in model organisms using BLAST.
You will put this on your website and also have a slide with this info when you present your work.
1. Find your primary literature article on your gene and disease on PubMed
2. Find your accession numbers and FASTA formats for your DNA and Protein via ENTREZ. You will hyperlink this data out.
3. Determine what Chromosome your gene is located on?
4. Get used to BLAST by going through a tutorial
5. Determine how similar your DNA/protein is to other genes/proteins in model organisms using BLAST.
You will put this on your website and also have a slide with this info when you present your work.
PubMed: http://www.ncbi.nlm.nih.gov/pubmed/
Entrez: http://www.ncbi.nlm.nih.gov/sites/gquery
Gene Cards: http://www.genecards.org/
UniProt: http://www.uniprot.org/
BLAST: http://blast.ncbi.nlm.nih.gov
Entrez: http://www.ncbi.nlm.nih.gov/sites/gquery
Gene Cards: http://www.genecards.org/
UniProt: http://www.uniprot.org/
BLAST: http://blast.ncbi.nlm.nih.gov
Things you should know
Hidden Markov Model
What is an E-value (Expect value)? The lower the E-value, or the closer it is to zero, the more "significant" the match is
Similarity: The extent to which nucleotide or protein sequences are related.
What is an E-value (Expect value)? The lower the E-value, or the closer it is to zero, the more "significant" the match is
Similarity: The extent to which nucleotide or protein sequences are related.
